Fig. 1

Evolution of single-cell RNA sequencing, workflow of 10 × scRNA-seq and spatial transcriptome. RNA ribonucleic acid, STRT-Seq single-cell tagged reverse transcription sequencing, MARS massively parallel RNA single cell, SMART Switching mechanism at 5’ end of the RNA transcript, Cell-seq cell expression by linear amplification and sequencing, SPLiT-Seq split-pool ligation-based transcriptome sequencing, sci-RNA-seq Single cell combinatorial indexing RNA sequencing, MATQ-Seq Multiple annealing and tailing-based quantitative single cell RNA sequencing, scAT-seq2 single-cell assay for transposase-accessible chromatin using sequencing 2, BART-seq barcode assembly for targeted sequencing, MULTI multiplexing using lipid-tagged indices for single-cell and single-nucleus RNA, HDST high-definition spatial transcriptomics, ScNaUmi-seq single-cell nanopore sequencing with unique molecular identifiers, tSCRB-seq T cell-tailored single-cell RNA barcoding and sequencing, Scifi single-cell combinatorial fluidic indexing