Fig. 7

Pretreatment with IFN-α can inhibit hepatic ferroptosis by stimulating DHX58. a DHX58 and GPX4 protein levels in the liver with IFN-α pretreatment and then I/R. Wild-type (WT) and Dhx58^hep/− mice were pretreated with IFN-α, and then underwent I/R, liver damage was examined by serum ALT and AST (b), liver pathology was analyzed by HE staining (c), necrotic area and Suzuki’s score were examined (d), ROS production was analyzed by DHE staining (e), hepatic iron (f) and LPO (g) levels were analyzed accordingly. Scale bar = 20 μm. Data are shown as mean ± SD (n = 4) or photographs from one representative of three independent experiments. ^*P < 0.05, ^**P < 0.01. ns non-significant, IFN-α interferon-α, DHX58 DExH-box helicase 58, GPX4 glutathione peroxidase 4, I/R ischemia/reperfusion, ALT alanine aminotransferase, AST aspartate aminotransferase, HE hematoxylin-eosin, ROS reactive oxygen species, DHE dihydroethidium, LPO lipid peroxide, SD standard deviation