Fig. 5
From: Interferon-α stimulates DExH-box helicase 58 to prevent hepatocyte ferroptosis
DHX58 associates Gpx4 mRNA and promotes its translation. a Schematic workflow of DHX58 downstream targets analysis. b The association between DHX58 and Gpx4 mRNA in primary hepatocytes were determined by RIP-qRT-PCR. *P < 0.05. c The top motif identified by HOMER of DHX58-bound peaks in Gpx4 mRNA. d The association between the CTD domain of DHX58 and endogenous Gpx4 mRNA in primary hepatocytes was determined by RIP-qRT-PCR. *P < 0.05, **P < 0.01, ns non-significant. e DHX58, ACSL4, COX2, SLC7A11, and GPX4 protein levels in liver tissues of Dhx58f/f and Dhx58hep−/− mice after I/R injury were examined by Western blotting. f DHX58, ACSL4, COX2, SLC7A11, and GPX4 protein levels in primary hepatocytes from Dhx58f/f and Dhx58hep−/− mice following H/R injury were examined by Western blotting. g GPX4 protein level in DHX58-overexpressed primary hepatocytes was examined by Western blotting. h DHX58, ACSL4, COX2, SLC7A11, and GPX4 protein levels in primary hepatocytes from Dhx58f/f or Dhx58hep−/− mice with GPX4 overexpression and treatment of H/R or erastin were examined by Western blotting. i In primary hepatocytes from Dhx58f/f and Dhx58hep−/− mice, relative Gpx4 mRNA distribution in each ribosome fractions was analyzed by qRT-PCR. Data are shown as mean ± SD (n = 3) or photographs from one representative of three independent experiments. #P < 0.05, ##P < 0.01 vs. Dhx58f/f. ▲CTD C-terminal domain deleted, DHX58 DExH-box helicase 58, GPX4 glutathione peroxidase 4, RIP RNA immunoprecipitation, HOMER hypergeometric optimization of motif enrichment, CTD C-terminal domain, ACSL4 acyl-CoA synthetase long chain family member 4, COX2 cyclooxygenase-2, SLC7A11 solute carrier family 7 member 11, I/R ischemia/reperfusion, H/R hypoxia/re-oxygenation, LMW low molecular weight, HMW high molecular weight, SD standard deviation