Fig. 3
From: Interferon-α stimulates DExH-box helicase 58 to prevent hepatocyte ferroptosis
Decreased DHX58 aggravates liver I/R injury. a DHX58 protein level in liver tissues and isolated hepatocytes from Dhx58f/f and Dhx58hep−/− mice was confirmed by Western blotting. Liver I/R injury was administrated in Dhx58f/f and Dhx58hep/− mice, liver injury was shown by gross appearances of representative livers, and white arrow indicated injured area (b), liver pathology was analyzed by HE staining (c), necrotic area and Suzuki’s score were analyzed (n = 4) (d), serum ALT and AST were examined (n = 4) (e), IL-6, IL-1β, and MCP1 mRNA levels in liver tissues were examined by qRT-PCR (n = 4) (f), infiltration of neutrophils and macrophages were analyzed by flow cytometry (g). Scale bar = 20 μm. Data are shown as mean ± SD or photographs from one representative of three independent experiments. *P < 0.05, **P < 0.01. DHX58 DExH-box helicase 58, I/R ischemia/reperfusion, HE hematoxylin-eosin, ALT alanine aminotransferase, AST aspartate aminotransferase, IL-6 interleukin-6, IL-1β interleukin-1β, MCP1 monocyte chemoattractant protein 1