Fig. 1

Hepatic DHX58 expression is markedly decreased during liver I/R injury. a Expression of hepatic DDX/DHX family members following liver I/R injury. b Immunofluorescence staining images of DHX58, hepatocyte and DAPI in liver sections analyzed by confocal microscopy. Scale bar = 50 µm. c DHX58 protein level in the isolated hepatocytes and non-parenchymal cells (NPCs) of the liver was examined by Western blotting. d DHX58 protein level in liver tissues after I/R injury was examined by Western blotting. e Dhx58 mRNA level in liver tissues after I/R injury was examined by qRT-PCR. f DHX58 protein level in primary hepatocytes following H/R injury was examined by Western blotting. g Dhx58 mRNA level in primary hepatocytes following H/R injury was examined by qRT-PCR. h DHX58 and COX2 in human normal liver tissues, obtained from distal normal liver tissues of liver hemangioma patients, and human liver tissues post I/R, obtained from biopsy approximately 3 h post reperfusion in donors during liver transplantation, were examined by Western blotting. The donor livers for transplantation underwent 5 to 10 min of warm ischemia followed by 5 to 7 h of cold ischemia. Quantified DHX58 expression was shown. i The correlation between DHX58 and COX2 in (h) was analyzed by Pearson’s correlation coefficient assay. Data are shown as mean ± SD (n = 3 or indicated) or photographs from one representative of three independent experiments. ^**P < 0.01 compared with the sham/control group by Student’s two-tailed t-test. DHX58 DExH-box helicase 58, I/R ischemia/reperfusion, DAPI 4’,6-diamidino-2-phenylindole, H/R hypoxia/re-oxygenation, COX2 cyclooxygenase-2, SD standard deviation