Fig. 2

Large platelets produced post IR are inherently hyperreactive. Flow cytometric analysis and quantification of the frequency of PMPs (a) and PMAs (b) in peripheral blood of mice at indicated dpi (n = 5). Flow cytometric quantification of CD62P expression (c) and GPIIb/IIIa activation (d) on washed platelets in response to thrombin at indicated dpi (n = 5). e Representative images and quantification of thrombus formation on collagen-coated flow chambers under shear-flow conditions using platelets obtained from mice at indicated dpi (n = 5). Scale bar = 250 μm. f, g GO and KEGG enrichment analysis of top upregulated signaling pathways in BM mMKs of mice at 1 and 3 dpi. Data represent mean ± standard deviation. ^**P < 0.01, compared to Ctrl. Two-tailed unpaired student’s t-test. Ctrl control, IR ionizing radiation, dpi day post IR, PMP platelet-derived microparticle, aPMP activated PMP, PMA platelet-monocyte aggregate, BSA bovine serum albumin, GO Gene Ontology, KEGG Kyoto Encyclopedia of Genes and Genomes, BM bone marrow, mMK mature megakaryocyte, ECM extracellular matrix, Jak Janus kinase, STAT signal transducer and activator of transcription