Fig. 1

mMKs are resistant to IR and produce large platelets post IR. a Platelet counts in peripheral blood of mice at indicated dpi (n = 10). b MPV in peripheral blood of mice at indicated dpi (n = 10). c Flow cytometric quantification of FSC-area of platelets in peripheral blood of mice at indicated dpi (n = 6). d, e Platelet-larger cell ratio and platelet size deviation width in peripheral blood of mice at indicated dpi (n = 10). f mMK numbers in BM of mice at indicated dpi (n = 6). g Representative hematoxylin and eosin staining of longitudinal sections of femurs of mice at indicated dpi. Scale bar = 40 μm. h Frequency of mMKs in BM of mice at indicated dpi (n = 6). i Frequency of MkPs in BM of mice at indicated dpi (n = 6). j Flow cytometric analysis and quantification of the frequency of nascent (thiazole orange⁺) platelets in peripheral blood of mice at indicated dpi (n = 6). k Flow cytometric quantification of FSC-area of thiazole orange⁺ platelets in peripheral blood of mice at indicated dpi (n = 6). l Viability of Meg-01 cells at 3 dpi as determined by Cell Counting Kit-8 (n = 5). m Flow cytometric analysis and quantification of FSC-area of PLPs in Meg-01 cultures at 3 dpi (n = 5). Data represent mean ± standard deviation. ^**P < 0.01, compared to Ctrl, two-tailed unpaired student’s t-test unless stated otherwise. ^#P < 0.05, ^##P < 0.01, compared to 15 dpi, one-way analysis of variance (k). ns non-significance, mMK mature megakaryocyte, Ctrl control, IR ionizing radiation, dpi day post IR, MPV mean platelet volume, FSC forward scatter, BM bone marrow, MkP megakaryocyte progenitor, PLP platelet-like particle