Fig. 3
Predominance of S100ahigh monocytes in late sepsis. a UMAP plot showed subclusters of circulating monocytes in mouse model of sepsis. b UMAP plots of monocyte subsets, across disparate sampling time points after CLP operation. c Histogram showed the relative expression of DEGs among all monocyte subpopulations. d Volcano plot compared upregulation of DEGs regarding mM03 vs. mM06. e Heatmaps displayed relative enrichment of each monocyte subcluster during the course of sepsis. f Curve plots showed proportion of mM03 (upper panel) and mM06 (lower panel) at distinct timepoints after CLP surgery. g The developmental trajectory of monocytes was colored-coded by the clusters and pseudo-time. Putative trajectory for cell transition states of monocyte, with proportion of each subcluster (upper right panel). UMAP uniform manifold approximation and projection, CLP cecal ligation and puncture, DEGs differentially expressed genes