Fig. 2

Enrichment of distinct monocyte subtypes in septic patients. a Histogram of unsupervised clustering analysis was divided enrolled participants into 6 clusters based on enrichment of disparate immune cell groups. b Quantitative bar charts were compared proportion of monocyte subsets across different clusters of individuals. c Heatmap showed relative expression level of top 5 DEGs among subclustered monocyte subsets. d Volcano plot displayed upregulation of DEGs regarding C08 vs. C10. e Bar graph listed the enriched biological processes in C08 (left panel) and C10 (right panel) by GO analysis. One-way ANOVA with Tukey HSD test was applied to calculate statistics. Data are shown as means ± SD. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001. DEGs differentially expressed genes, Mono monocyte, DC dendritic cell, NK natural killer, ANOVA analysis of variance, SD standard deviation