Fig. 1

Single-cell atlas of PBMCs from septic patients. a Schematic workflow of the current study. b UMAP visualization of scRNA-seq profiles of 48 samples (29 septic patients and 19 HC) displayed the annotation and color codes for 18 immune cell subclusters. c Violin plots indicated expression level of canonical annotation marker gene. d Proportion and absolute counts of each subcluster and each immune cell type across enrolled participants. e Quantitative bar charts showed the comparison of percentage of each subcluster and each immune cell type between patients with sepsis and HC. Statistics were analyzed by unpaired two-sided Student’s t test. Data are shown as means ± SD. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001. PBMCs peripheral blood mononuclear cells, UMAP uniform manifold approximation and projection, scRNA-seq single-cell RNA sequencing, HC healthy controls, SD standard deviation, Mono monocyte, DC dendritic cell, NK natural killer