Fig. 5

Lf3 and SAHA inhibits ESCC cell growth and β-catenin/c-Myc signaling, and melatonin co-treatment further enhances these actions. a and c Representative images and results of colony formation assay with Lf3 + melatonin or SAHA + melatonin pretreatments for 48 h in the EC109 and EC9706 cells. b and d Co-treatment of Lf3 + melatonin or SAHA + melatonin was applied to EC109 and EC9706 cells for 48 h. Representative Western blotting results of HDAC7, c-Myc, phosphorylated β-catenin (Ser675), acetylated β-catenin (Lys49), β-catenin, p21 and p27 were shown. ^*P < 0.05 vs. the Lf3 0 μmol/L group + MEL 0 mmol/L group, ^#P < 0.05 vs. the Lf3 0 μmol/L group + MEL 4 mmol/L group, ^$P < 0.05 vs. the only Lf3 treatment groups, ^&P < 0.05 vs. the only SAHA treatment groups. HDAC7 histone deacetylase 7, MEL melatonin