Fig. 6

C3 and C4 diminish the lytic activity of osteoclasts. Pre-osteoclasts were cultured in the presence of C3 (5 mmol/L) or C4 (1 mmol/L) with IL-1β (40 ng/ml) for 5 d. Relative expression levels of bone resorption-related mRNA (a) and proteins (b) by osteoclasts. c Quantification of osteoclast bone resorption-related proteins. d Phalloidin labelling of F-actin in osteoclasts cultured on glass coverslips. Results are mean ± SEM, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. C3 propionate, C4 butyrate, IL-1β interleukin 1 beta, RANKL receptor activator of NF-κB ligand, TRAP tartrate resistant acid phosphatase, MMP9 matrix metalloprotein 9, CTSK cathepsin K