Fig. 2

C3 and C4 inhibit NLRP3 inflammasome activation by CoCrMo alloy particles. BMDMs (LPS-primed) were treated with C3 or C4 and then stimulated with CoCrMo alloy particles. a Supernatants were collected for measuring IL-1β and IL-18 release by ELISA. b, c BMDMs (LPS-primed) were treated with different doses of C2, C3, C4, and then stimulated with CoCrMo alloy particles. Cell lysates and supernatants were analysed by immunoblotting. d LPS-primed THP-1 macrophages (PMA-differentiated) treated with C2, C3, C4, and then stimulated with CoCrMo alloy particles. Supernatants were analysed by immunoblotting. Results are mean ± SEM, ^***P < 0.001, ns non-significant. BMDMs bone marrow-derived macrophages, C2 acetate, C3 propionate, C4 butyrate, IL-1β interleukin 1 beta, IL-18 interleukin 18, LPS lipopolysaccharide