Fig. 2

TCI induces acute dysfunctions of the rat intestine. a ELISA to detect indicator concentrations of rats in the naïve (n = 15), sham (n = 15) and TCI (n = 13) groups. b IHC analysis to detect ZO-1 and occludin expression in the intestinal tissues at distances of 1 cm, 3 cm and 5 cm from the lesions. The histograms of IHC scores are on the right of IHC pictures. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001, ns non-significant. TCI traumatic colon injury, IL-6 interleukin-6, TNF-α tumor necrosis factor-α, DAO diamine oxidase, ZO-1 zonula occludens 1, IHC immunohistochemical, ELISA enzyme-linked immunosorbent assay