Fig. 8
From: Celastrol mitigates inflammation in sepsis by inhibiting the PKM2-dependent Warburg effect
Celastrol (Cel) attenuates lipopolysaccharide (LPS)-induced inflammatory response and glycolysis. a Transfection of macrophages with short interfering (si)RNA against PKM2, and Western blotting with densitometry (n = 3). *P < 0.05 vs. si-NC. b IL-1β levels in cells transfected with si-PKM2 or si-NC and cultured in the presence or absence of Cel at 0.5 μmol/L (n = 3). #P < 0.05 vs. DMSO; **P < 0.01 vs. si-NC. c ECAR of cells transfected with si-PKM2 in the presence or absence of Cel at 0.5 μmol/L (n = 3). ###P < 0.001 vs. DMSO; *P < 0.05, **P < 0.01, ***P < 0.001 vs. Model. PKM2 pyruvate kinase M2, IL-1β interleukin-1β, ECAR extracellular acidification rate, 2-DG 2-deoxy-glucose, NC negative control