Fig. 6
From: Celastrol mitigates inflammation in sepsis by inhibiting the PKM2-dependent Warburg effect
Celastrol (Cel) binds to Cys residues in PKM2, LDHA and HMGB1. a Recombinant human PKM2 labeled by celastrol-probe (Cel-P) or alkyne-tagged IAA in the presence or absence of the competitors Cel or IAA. b Recombinant human LDHA labeled by celastrol-probe (Cel-P) or alkyne-tagged IAA in the presence or absence of the competitors Cel or IAA. c Recombinant human HMGB1 labeled by celastrol-probe (Cel-P) or alkyne-tagged IAA in the presence or absence of the competitors Cel or IAA. d Recombinant human HMGB1 A box labeled by celastrol-probe (Cel-P) or alkyne-tagged IAA in the presence or absence of the competitors Cel or IAA. e Recombinant human HMGB1 B box labeled by celastrol-probe (Cel-P) or alkyne-tagged IAA in the presence or absence of the competitors Cel or IAA. PKM2 pyruvate kinase M2, LDHA lactate dehydrogenase A, HMGB1 high mobility group box 1, Conc. concentration, Fluo fluorescence, CBB coomassie brilliant blue, IAA iodoacetamide