Fig. 5
From: Microfluidics-based strategies for molecular diagnostics of infectious diseases
CRISPR-based methods for nucleic acid testing. a Integrated CRISPR-based molecular diagnostic centrifugal POCT (adapted from [92]). b Development of the CASCADE assay for smartphone-based SARS-CoV-2 detection (adapt from [93]). RAA recombinase-aided amplification, PAM protospacer adjacent motif, CRISPR clustered regularly interspaced short palindromic repeats, CASCADE Cellphone-Based Amplification-Free System with CRISPR/CAS-dependent enzymatic, EDC 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride