Fig. 2
From: Small molecules facilitate single factor-mediated sweat gland cell reprogramming
Acquisition of SG cells phenotype by forced transgenic expression of EDA and SGM culture. a Phase contrast images showing the morphological changes of HF-EDA in SGM on day 0, 3, 10 and 15. Scale bar = 200 μm. Illustrations, higher magnification of the boxed areas. b qPCR analysis of transcriptional expression of CK5, CK10, CK18, CEA and AQP5 in HDF and HDF-EDA cultured in SGM after 15 d of induction. c, d Flow cytometry quantification of CK5 positive cells and CK18 positive cells of HDF-EDA in SGM. e qPCR analysis of EGFR and FGFR2 mRNA levels in HDF-EDA cultured in SGM. n = 3. Data were expressed as mean ± SD and analyzed by two-tailed t-tests, *P < 0.05, **P < 0.01, ***P < 0.001, ns not significant, SG sweat gland, SGM sweat gland culture medium, EDA ectodermal dysplasia antigen, CK5 cytokeratin 5, CK10 cytokeratin 10, CK18 cytokeratin 18, CEA carcino-embryonic antigen, AQP5 aquaporin 5, HDF human dermal fibroblasts, EGFR epidermal growth factor receptor, FGFR2 fibroblast growth factor receptor 2