Fig. 8

UTMD induced radiosensitization of glioblastoma cells in a PGRMC1-dependent manner. GL261 and U251 cells were transfected with lentiviral vectors encoding PGRMC1 or PGRMC1 siRNA. Then, the cells were treated with UTMD followed by IR (2 Gy) exposure. a Cell viability was assessed with a CCK-8 detection kit. b Cell death was detected by PI staining followed by flow cytometry. GL261 and U251 cells were pretreated with AG-205 (10 μmol/L) for 1 h and then cells were treated with UTMD followed by IR (2 Gy) exposure. c Cell viability was assessed with a CCK-8 detection kit. d Cell death was detected by PI staining followed by flow cytometry. Values are expressed as mean ± SD (n = 3). *P < 0.05 vs. the vehicle-treated control group; ^#P < 0.05, ^##P < 0.01 vs. IR group; ^&P < 0.05, ^&&P < 0.01 vs. IR plus UTMD group. IR ionizing radiation, UTMD ultrasound-triggered microbubble destruction, PGRMC1 progesterone receptor membrane component 1, CCK-8 cell counting kit-8, PI propidium iodide, SD standard deviation