Fig. 6

UTMD inhibited PGRMC1 expression and binding with LC3B in IR-treated glioblastoma cells. a, b GL261 and U251 cells were treated with UTMD-L (ultrasonic intensity of 1.2 W/cm² combined with 100 μl/ml MBs) or UTMD-H (ultrasonic intensity of 1.2 W/cm² combined with 200 μl/ml MBs), and then the cells were exposed to IR (2 Gy). PGRMC1 expression was measured by Western blotting. c GL261 and U251 cells were pretreated with UTMD. Then, the cells were exposed to IR (2 Gy) and incubated for an additional 24 h. Immunofluorescence staining analysis of PGRMC1 (green) and LC3B (red) expression, the nuclei were stained with DAPI (blue). Values are expressed as mean ± SD (n = 3). *P < 0.05 vs. the vehicle-treated control group; ^##P < 0.01 vs. IR group. IR ionizing radiation, UTMD ultrasound-triggered microbubble destruction, PGRMC1 progesterone receptor membrane component 1, ACTB β-actin, LC3B light chain 3 beta, MBs microbubbles, SD standard deviation, a.u. arbitrary units