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Fig. 1 | Military Medical Research

Fig. 1

From: Melatonin attenuates radiation-induced cortical bone-derived stem cells injury and enhances bone repair in postradiation femoral defect model

Fig. 1

Effect of melatonin on self-renewal and multi-directional differentiation potential of postradiaiton CBSCs. a Protocols for extracting CBSCs. b Identification of CBSCs by flow cytometry. c, d Colonies count for detecting self-renewal ability. e qRT-PCR analysis of osteogenic and adipogenic gene markers. f ALP staining and Alizarin red staining after osteogenic induction. Oil red O staining after adipogenic induction. Compared with control, *P < 0.05; compared with IR, #P < 0.05. IR irradiation, IR + LMLT irradiation plus low 1 μmol/L melatonin treatment, IR + MMLT irradiation plus medium 10 μmol/L melatonin treatment, IR + HMLT irradiation plus high 100 μmol/L melatonin treatment, CBSCs cortical bone-derived stem cells, ALP alkaline phosphatase

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Military Medical Research

ISSN: 2054-9369