Fig. 3

The molecules involved in RIG-I signaling in the CS-AKI cell model. a–c. qPCR, and Western blotting analyses the RIG-I expression after NRK-52E cells treatment with 1, 3 or 6 μg/ml Poly I:C for 24 h respectively. Fig. c is the quantification of Fig. b. d. qPCR analyses RIG-I expression activated by different concentrations of ferrous myoglobin. e–f. qPCR analyses cells KIM-1 and NGAL expression after treatment with 200 μmol/L ferrous myoglobin at 6 h and 24 h separately. g-i. qPCR, and Western blotting analyses RIG-I expression. Fig. i is the quantification of Fig. h. j–l. qPCR, and Western blotting analyses IRF3 and p-IRF3 expression. Fig. l is the quantification of Fig. k. m. qPCR analyses IFN-β expression. n. Cell supernatant levels of IFN-β by ELISA. o–q. qPCR, and Western blotting analyses P65 and p-P65 expression. Fig. q is the quantification of Fig. p. r–t. qPCR, and Western blotting analyses caspase-3 and cleaved caspase-3 expression. Fig. t is the quantification of Fig. s. Data are expressed as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, two-way ANOVA followed by the Bonferroni’s multiple comparisons test or one-way ANOVA followed by the Brown–Forsythe multiple comparisons test, respectively