Fig. 2

The expressions of downstream molecules involved in RIG-I signaling in the CS-AKI rat model. IRF3: Interferon regulatory factor 3. a–c. qPCR, and Western blotting analyses expression levels of renal IRF3. Fig. c is the quantification of Fig. b. d. qPCR analyses of renal IFN-β expression. e. Serum levels of IFN-β by ELISA. f–h. qPCR, and Western blotting analyses renal P65 and p-P65 expression. Fig. h is the quantification of Fig. g. i–j. IHC analyses renal IRF3, p-IRF3, P65, and p-P65 expression after relieving the pressure at 12 h and 36 h (scale bar: 100 μm). Fig. i is the quantification of IHC staining. k–m. qPCR, and Western blotting analyses renal caspase-3 and cleaved caspase-3 expression. Fig. m is the quantification of Fig. l. n–o. IHC analyses renal caspase-3 and cleaved caspase-3 expression (scale bar: 100 μm), Fig. n is the quantification of Fig. o; Data are expressed as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, two-way ANOVA followed by the Bonferroni’s multiple comparisons test