Fig. 6
Effects of DIRAS3 and p53 re-expression on tumor growth in vivo. CAL-27 xenograft-bearing nude mice were intratumorally injected every 3 days with Ad-GFP, Ad-DIRAS3, rAd-p53, or Ad-DIRAS3 plus rAd-p53 (No. of mice for each group = 5). Nude mice were treated with 4 cycles of adenovirus injection in 12 days. Tumor diameters were measured every 6 days, and the volume was calculated using the following formula: π/6 × larger diameter×(smaller diameter)2. At 36 days after the end of adenovirus injection, mice were sacrificed, and tumors were excised. Each tumor in four groups was shown, and the tumor volume was calculated as shown in (a). The tumor volume at the endpoints of the experiment in each group was as follow: Control group: 189.02 ± 17.54 mm3, DIRAS3 group: 8.45 ± 1.73 mm3, p53 group: 29.99 ± 8.38 mm3, DIRAS3 plus p53 group: 3.12 ± 0.75 mm3. The tumor volume at different time points was plotted in (b). Relative mRNA levels of p21WAF1/CIP1, Bax and Bcl-2 in tumor tissue homogenates were examined by real-time PCR (c). Heart, lung, liver, kidney and spleen sections were stained with hematoxylin and eosin (H&E) for histopathological analysis (d). *P < 0.05; **P < 0.01; ***P < 0.001