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Fig. 5 | Military Medical Research

Fig. 5

From: Efficient and rapid conversion of human astrocytes and ALS mouse model spinal cord astrocytes into motor neuron-like cells by defined small molecules

Fig. 5

Direct conversion of spinal-cord astrocytes isolated from the adult amyotrophic lateral sclerosis (ALS) mouse model into MN-like cells. a Representative images of spinal-cord astrocytes from adult ALS mice (ALS-As). b Immunostaining of GFAP expression in ALS-As. c Representative images of ALS-As-induced motor neuron-like cells (iMNs) (ALS-As-iMNs). d Immunostaining of the neuronal markers TUJ1 and MAP2 and the MN-specific markers HB9 and ISL1 in ALS-As-iMNs. All scale bars = 50 μm. e The percentage of TUJ1+ cells compared to the total number of DAPI+ cells (mean ± SEM, n = 10 randomly selected 20× fields from triplicate samples). f The percentage of TUJ1+HB9+ cells to the total number of DAPI+ cells (mean ± SEM, n = 10 randomly selected 20× fields from triplicate samples)

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Military Medical Research

ISSN: 2054-9369