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Fig. 4 | Military Medical Research

Fig. 4

From: Efficient and rapid conversion of human astrocytes and ALS mouse model spinal cord astrocytes into motor neuron-like cells by defined small molecules

Fig. 4

The gene expression profiles and electrophysiological properties of hiMNs. a RT-qPCR analysis of mRNA expression levels of genes HB9, ISL1, SMN, NEUROD1, NGN2, MYT1, and GFAP during chemical induction. The values are presented as the means ± SEM (n = 3. *P < 0.05. **P < 0.01. ***P < 0.001. versus day 0. b Current-clamp recordings of hiMN generated from human astrocytes after chemical induction, showing action potentials in response to a depolarizing step current from − 60 to 120 pA (n = 6/10, recorded cells). c Tetrodotoxin (TTX) could inhibit action potentials. d Representative traces of whole-cell current in voltage-clamp mode, showing inward sodium current and outward potassium current (n = 5/7, recorded cells). e An inward sodium current that was blocked by TTX

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Military Medical Research

ISSN: 2054-9369