Fig. 3
From: PM2.5 obtained from urban areas in Beijing induces apoptosis by activating nuclear factor-kappa B
Effects of PM2.5 on NF-κB activity in CHO cells. a. The fluorescent cell images (NF-κB activation) obtained from an IN Cell Analyzer 1000 system. The nucleus is shown in blue and the cytoplasm in green. b. The effects of PM2.5 on NF-κB activity in the NF-κB activation responsive d2EGFP reporter system. CHO cells were seeded on 96-well plates at 4.0 × 104 cells/ml in 200 μl F12-K medium supplemented with 10% FBS without antibiotics and incubated for 18 h until the cells reached 90–95% confluency. The cells were transfected and treated with 100, 200, and 400 μg/ml PM2.5. An IN Cell Analyzer 1000 system was used to obtain and analyze images of the cells every half an hour. Data are expressed as the mean ± SD. Each data point represents an average of 3 samples