The roles of carboxylesterase and CYP isozymes on the in vitro metabolism of T-2 toxin

Table 2 Different inhibition effects of CYP450 isozymes on T-2 toxin metabolism in HLMs

Isozyme	Inhibitor	Percent of remaining T-2 toxin without BNPP (%)	Percent of remaining T-2 toxin with BNPP (%)
CYP2C19	Trans-2-phenylcyclopropylamine	0.19 ± 0.08	107.24 ± 3.16
CYP3A4	Ketonazle	0.27 ± 0.06	112 ± 6.10
CYP 2C9	Sulfaphenazole	0.10 ± 0.04	102.10 ± 0.80
CYP 2D6	Quinidine	0.26 ± 0.06	96.87 ± 7.87
CYP 1A2	α-naphthol	0.14 ± 0.08	99.37 ± 2.68
Negative control	No inhibitor	0.16 ± 0.07	97.10 ± 8.34
Control^a	No inhibitor	100 ± 3.86	100 ± 3.86

^aThe termination solution was added at the beginning in HLMs and no NADPH added to active the reaction

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