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Table 2 Different inhibition effects of CYP450 isozymes on T-2 toxin metabolism in HLMs

From: The roles of carboxylesterase and CYP isozymes on the in vitro metabolism of T-2 toxin

Isozyme Inhibitor Percent of remaining T-2 toxin without BNPP (%) Percent of remaining T-2 toxin with BNPP (%)
CYP2C19 Trans-2-phenylcyclopropylamine 0.19 ± 0.08 107.24 ± 3.16
CYP3A4 Ketonazle 0.27 ± 0.06 112 ± 6.10
CYP 2C9 Sulfaphenazole 0.10 ± 0.04 102.10 ± 0.80
CYP 2D6 Quinidine 0.26 ± 0.06 96.87 ± 7.87
CYP 1A2 α-naphthol 0.14 ± 0.08 99.37 ± 2.68
Negative control No inhibitor 0.16 ± 0.07 97.10 ± 8.34
Controla No inhibitor 100 ± 3.86 100 ± 3.86
  1. aThe termination solution was added at the beginning in HLMs and no NADPH added to active the reaction