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Figure 1 | Military Medical Research

Figure 1

From: First imported Plasmodium ovale malaria in Central America: case report of a Guatemalan soldier and a call to improve its accurate diagnosis

Figure 1

Molecular detection of Plasmodium ovale in blood samples from patient by the amplification of 18S gene. Nested PCR with Plasmodium genus-conserved primers was followed by P. ovale specific primers. PCR products were separated by electrophoresis through a 2.0% agarose gel stained with ethidium bromide and flanked by a 50 bp DNA ladder (Novagen) as a size marker. Lane MM: Molecular marker 50 bp. Lane TBF: Patient’s thin blood film. LaneTBF-2: Patient’s thin blood film (duplicate). Lane WB: Patient’s whole blood. Lane Pf : Plasmodium falciparum control. Lane Pv : Plasmodium vivax control. Lane Pm : Plasmodium malariae control. Lane Po: Plasmodium ovale positive control. Lane C-: Negative control.

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