Molecular detection of
in blood samples from patient by the amplification of 18S gene. Nested PCR with Plasmodium genus-conserved primers was followed by P. ovale specific primers. PCR products were separated by electrophoresis through a 2.0% agarose gel stained with ethidium bromide and flanked by a 50 bp DNA ladder (Novagen) as a size marker. Lane MM: Molecular marker 50 bp. Lane TBF: Patient’s thin blood film. LaneTBF-2: Patient’s thin blood film (duplicate). Lane WB: Patient’s whole blood. Lane
Plasmodium falciparum control. Lane
Plasmodium vivax control. Lane
Plasmodium malariae control. Lane Po:
Plasmodium ovale positive control. Lane C-: Negative control.