Fig. 3
From: Applications and prospects of cryo-EM in drug discovery
Application of cryo-EM in fragment-based drug design (FBDD). a Schematic diagram of FBDD technology. b Cryo-EM 3D map of β-galactosidase (Bgal) colored by chain. The active sites are marked by black box and displayed in different color. Structures of β-D-thiogalactopyranoside (PETG), L-ribose and 1-deoxy-glactonojirimycin (DGN) fit to the density maps of the Bgal complexes (PDB: 6TTE, 6TSK, 6TSH. EMDB: EMD-10574, EMD-10564, EMD-10563). The molecules are colored differently according to the active sites, respectively. c Cryo-EM 3D map of pyruvate kinase isozymes M2 (PKM2) colored by chain. The compound binding sites are marked by box and displayed in different color. Structures of L-theronine, compound 5, compound 6 and compound 10 fit to the density maps of the PKM2 complexes (PDB: 6TTH, 6TTF, 6TTI, 6TTQ. EMDB: EMD-10576, EMD-10575, EMD-10577, EMD-10584). The molecules are colored differently according to the cavities