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Fig. 5 | Military Medical Research

Fig. 5

From: Pericytes protect rats and mice from sepsis-induced injuries by maintaining vascular reactivity and barrier function: implication of miRNAs and microvesicles

Fig. 5

PCMVs carry miR-145 and miR-132 to play coordinated effects on the VSMCs and VECs. a Effects of different types of PCMVs on contractile response of rat VSMC to NE after LPS administration (n = 8 cells). b Western blotting analysis of p-MLC20, Sphk2, S1PR1 and S1PR2 from VSMCs treated with different types of PCMVs (n = 3 cells). c Different types of PCMVs were added into rat VECs, and FITC–BSA penetration of each group was measured (n = 8 cells). d Western blotting analysis of ZO-1, VE-cadherin, Sphk2, S1PR1 and S1PR2 from VECs treated with different types of PCMVs (n = 3 cells). e–f Western blotting analysis of p-MLC20, ZO-1 and VE-cadherin from VSMCs and VECs with S1PR1 inhibition (W146) and S1PR2 inhibition (JTE013) (n = 3 cells). LPS lipopolysaccharides, PC pericyte, PCMV pericyte-derived microvesicle, VECs vascular endothelial cells, VSMCs vascular smooth muscle cells, Sphk2 sphingosine kinase 2, S1PR1 sphingosine-1-phosphate receptor 1, p-MLC20 phosphorylation of myosin light chain 20, ZO-1 zonula occludens-1, VE-cadherin vascular endothelial cadherin, Ad-SK2 adenovirus-mediated overexpression of Sphk2, Negative infection of VSMCs or VECs with negative control adenovirus. Data shown as mean ± SD. ***P < 0.001 vs. Normal control; ##P < 0.01, ###P < 0.001 vs. LPS; $$P < 0.01 vs. LPS + PCMV; @@P < 0.01 vs. Ad-SK2 (one-way ANOVA)

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