Fig. 5
From: Data analysis guidelines for single-cell RNA-seq in biomedical studies and clinical applications
Two main types of metabolic analysis within scRNA-seq: pathway-based functional enrichment analysis and flux balance analysis of metabolic flow. While the former makes use of standard functional enrichment analysis, and the latter utilizes constraint-based mathematical models to systematically simulate metabolism in metabolic networks. Methods including scFBA, Compass, and scFEA employed different implementation strategies for flux balance analysis of metabolic flow. FBA flux balance analysis, KEGG Kyoto Encyclopedia of Genes and Genomes, UMAP uniform manifold approximation and projection, scRNA-seq single-cell RNA sequencing, scFBA single-cell flux balance analysis, scFEA single-cell flux estimation analysis, PCA principal component analysis