Fig. 6

Relationship between ENST00000547281 and CDK4/6 inhibitor sensitivity. a Scatter diagrams of the palbociclib/ribociclib IC₅₀ values and CDK4 gene and CDK4 transcript ENST00000547281 levels. b Splicing pattern of all CDK4 transcripts, the sequencing plot is part of the sanger sequencing of plasmid vectors carrying CDK4 transcripts ENST00000257904 and ENST00000547281, and the black box is the translation start site of ENST00000547281. In the structure plot and sequencing results, it can be seen that the sequence of ENST00000547281 transcript is part of ENST00000257904 transcript. c The histogram shows the relative expression levels of ENST00000257904 and ENST00000547281 by plasmid transfection into MCF7 cells, the left side is the ENST00000257904 plasmid group, and the right side is the ENST00000547281 plasmid group. d Line graph shows the proliferation and drug toxicity curves of MCF7 cells. The X-axis is the time after the cells were seeded into the plates, and the Y-axis is cell density index normalized by that at 0 h. NC negative control, GDSC Genomics of Drug Sensitivity in Cancer