Fig. 3

Expression patterns and characteristics of periodic transcripts in HeLa cervical cancer cells, HCT116 colon cancer cells and MDA-MB-231 breast cancer cells. a The rows show the normalized FPKM values of periodic transcripts to visualize their dynamic changes during the cell cycle. Each column represents a cell sample. They are samples at consecutive time points after cell cycle synchronization in G₁/S phase. From left to right are samples in each phase of G₁/S-S-G₂/M-G₁. Each row represents a transcript, and the transcripts from top to bottom are the transcripts highly expressed in each phase of G₁/S-S-G₂/M-G₁. b Overall distribution of FPKM values for all the periodic transcripts in HeLa, HCT116 and MDA-MB-231 cells. c Bar plot shows the proportion of major types of periodic transcripts (the proportion is more than 1%). The reference is the collection of all expressed periodic and aperiodic transcripts to compare the enrichment of transcript types, the types of periodic transcripts in the figure are not significantly enriched. d Bar plot shows the proportion of the main types of genes periodic transcripts belong to (the proportion is more than 1%), and the reference is the collection of all expressed genes. Among the protein-coding gene type, the proportion of genes to which periodic transcripts belong is higher than that of the reference, indicating that the periodic transcripts were enriched to protein-coding genes. FPKM fragments per kilobase of exon model per million mapped fragments