Fig. 2
Effects of I/R and PIASy shRNA on PIASy, Nav1.5, and Cav-3 expression. a H/R-induced changes in PIASy, Nav1.5, Cav-3, and SUMO2/3 expression levels in isolated cardiomyocytes from the left ventricle of adult rats (arrows). PIASy (red) and SUMO2/3 (green) fluorescence were detected at high levels, while Nav1.5 (green) and Cav-3 (red) showed decreased localization, especially on membrane. b I/R and PIASy shRNA altered PIASy, Nav1.5 and Cav-3 mRNA levels in rat left ventricle tissues by qRT-PCR. c–e PIASy, Nav1.5, and Cav-3 protein expression in whole cardiomyocytes (c), membrane (d) and cytoplasmic (e) fractions after I/R in Scramble-shRNA and PIASy-shRNA groups. The left panel represents Western blot bands while the right represents their densitometric analysis. Values are expressed as the mean ± standard error of the mean (n = 10). *P < 0.05, **P < 0.01, one-way ANOVA. ANOVA analysis of variance, Cav-3 caveolin-3, H/R hypoxia/reoxygenation, I/R ischemia/reperfusion, Nav1.5 voltage-gated sodium channel 1.5, PIASy protein inhibitor of activated STAT Y, qRT-PCR quantitative real-time polymerase chain reaction, shRNA short hairpin RNA