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Fig. 2 | Military Medical Research

Fig. 2

From: Melatonin inhibits ESCC tumor growth by mitigating the HDAC7/β-catenin/c-Myc positive feedback loop and suppressing the USP10-maintained HDAC7 protein stability

Fig. 2

High HDAC7 expression predicts poor prognosis of ESCC patients and melatonin inhibits ESCC proliferation via inhibiting HDAC7. a Representative IHC images for HDAC7 expression in ESCC tissues. Scale bar, 200 μm and 50 μm (inset), respectively. b Statistical analysis of HDAC7 expression in 148 ESCC patients through IHC staining. c Kaplan–Meier survival analysis by the high/low HDAC7 levels of the 148 ESCC patients based on the microarray tissue IHC results. d Representative HDAC7 protein level in different ESCC cell lines. e Photographs and results showing xenograft tumor morphologies and weights in each group after subcutaneously injection of NC or HDAC7 OE EC109 cells, respectively. f Representative images and results of colony formation and EdU incorporation assays. g Representative Western blotting results of HDAC7, c-Myc, p21, p27 and Cyclin D1. *P < 0.05 vs. the NC group or shCon group, #P < 0.05 vs. the MEL 4 mmol/L group, $P < 0.05 vs. the HDAC7 OE or shHDAC7 group. HDAC7 histone deacetylase 7, ESCC esophageal squamous cell carcinoma, MEL melatonin, OE overexpression, IHC immunohistochemistry

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Military Medical Research

ISSN: 2054-9369