Fig. 6
On-chip investigation of the drug (combination of Ara-C and DNR) effects on single cells isolated from AML patient bone marrow species. a Work flow showing the sample collection, centrifuge-based cell separation, anti-CD34 antibody labelled magnetic beads-based purification, and the on-chip assay. b High-throughput and high-efficiency capture of the primary cells with the modified MAC. The inset shows that the compacted microchamber perfectly matches the size of primary AML cells. The scale bar is 200 μm for b and 100 μm for the inset. c Representative microchambers in the channels 1, 3 and 5 showing the live/dead cells of patient sample 1 after Ara-C/DNR treatment for 24 h. The drug concentrations in channels 1, 3 and 5 are 0, 4.21/0.84 and 8.40/1.68 μmol/L, respectively. Scale bar = 100 μm. d Heatmap showing the live cells of patient sample 1 in the representative microchambers after drug treatment. The drug concentrations in channels 1 − 6 are 0, 2.11/0.42, 4.21/0.84, 6.31/1.26, 8.40/1.68 and 10.0/2.0 μmol/L for Ara-C/DNR, respectively. e Normalized cell proliferation rates of the two AML patient samples in the 6 channels with different drug concentrations. P1, P2 indicate patient number