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Fig. 5 | Military Medical Research

Fig. 5

From: An integrated microfluidics platform with high-throughput single-cell cloning array and concentration gradient generator for efficient cancer drug effect screening

Fig. 5

Combined drug treatment on single K562 cell-derived clones in MAC. a Representative area showing single cell captured (t = 0 h), cultured (t = 48 h) and Ima/Res treated for 24 h (t = 72 h) in the channels 1, 3 and 5. The drug concentrations in channel 1, 3 and 5 are 0, 3.36/105.15 and 6.72/210.10 μmol/L. After drug treatment, calcein-AM/PI were used to identify cell viability. Scale bar = 100 μm. b Normalized cell proliferation rate of single clones in representative microchambers after combined drug stimulation (t = 72 h). Drug concentrations of Ima/Res in channels 1−6 are 0, 1.69/52.68, 3.36/105.15, 5.04/157.63, 6.72/210.10 and 8/250 μmol/L respectively. c Normalized cell proliferation rate across the device under the three drug treatments. The maximum drug concentration in each treatment is indicated

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Military Medical Research

ISSN: 2054-9369