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Fig. 4 | Military Medical Research

Fig. 4

From: An integrated microfluidics platform with high-throughput single-cell cloning array and concentration gradient generator for efficient cancer drug effect screening

Fig. 4

Single or combined drug treatment with Imatinib (Ima) and Resveratrol (Res) on single K562 cells captured in MAC. a Selected areas in the first (channel 1), third (channel 3) and fifth (channel 5) channel showing the single cells at t = 0 h and 24 h with the treatment of combined drugs Ima/Res. The drug concentrations in channel 1, 3 and 5 are 0, 0.84/63.09 and 1.68/126.06 μmol/L, respectively. After drug treatment, calcein-AM/PI were used to identify cell viability. Scale bar = 100 μm. b Live cells in the representative microchambers after Ima, Res and Ima/Res treatment. The drug concentrations in channels 1 − 6 are 0, 0.63, 1.26, 1.89, 2.52 and 3.00 μmol/L for Ima, and 0, 63.21, 126.18, 189.15, 252.12 and 300.00 μmol/L for Res, and 0, 0.42/31.61, 0.84/63.09, 1.26/94.58, 1.68/126.06 and 2/150 μmol/L for Ima/Res, respectively. c Overall cell proliferation rates under different drug treatments in the 6 channels with different drug concentrations. The sixth channel with the maximum drug concentration was indicated

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