Fig. 2

Evaluation of the efficiency of single-cell capture and single-cell cloning on the devices. a Capture rate in the single-plexed device with different cell densities. Scale bar = 200 μm. b Typical single-cell capturing micrograph showing the 4320 microchambers with about 3000 single cells captured in the array. Scale bar = 400 μm. c Time-lapse images of the same chambers showing single-cell capture, cloning and staining. Scale bar = 50 μm. d Statistics of the frequency distribution (%) of cell numbers in single K562 cell-derived clones’ experiment on MAC. The numbers indicate the cell numbers of different single-cell derived clones at t = 72 h. e Investigation of the relationship between cell division rate and initial cell size in some representative microchambers