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Fig. 3 | Military Medical Research

Fig. 3

From: Propionate and butyrate attenuate macrophage pyroptosis and osteoclastogenesis induced by CoCrMo alloy particles

Fig. 3

C3 and C4 block the assembly of ASC and pyroptosis stimulated by CoCrMo alloy particles in macrophages. a Representative immunofluorescence images of ASC speck formation in BMDMs (LPS-primed) stimulated with CoCrMo alloy particles in the presence of C3 or C4. b Quantification of ASC speck formation. BMDMs (LPS-primed) were treated with C3 (c) or C4 (d) and then stimulated with CoCrMo alloy particles. ASC oligomerization in cross-linked cytosolic pellets was analysed and GSDMD was detected by immunoblotting. ASC apoptosis-associated speck-like protein containing a caspase recruitment domain, GSDMD gasdermin D, GSDMD-NT gasdermin D-N-terminal fragment, C3 propionate, C4 butyrate, LPS lipopolysaccharide

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Military Medical Research

ISSN: 2054-9369