Fig. 6

Asn plus IPA regimen facilitates intestinal homeostasis recovery by activating Akt signaling in the intestinal epithelium. a The pie charts to show the proportions of protein phosphorylation sites that are enriched in PI3K/Akt pathway among the sites with significantly altered phosphorylation levels (FC ≥ 1.3 or FC ≤ 0.7). b The antibody microarray images to show the landscape of detected protein expression and phosphorylation. Akt Thr308 phosphorylation levels are compared between vehicle, Asn and IPA groups. c Western blotting analysis to show protein expression and phosphorylation of intestinal epithelium of rats that received indicated treatment. d The flow diagram to depict the experimental processes of preparing rats with TCI that were administered with vehicle, MK-2206, Asn plus IPA or Asn plus IPA and MK-2206 (n = 15 per group). e The survival curve to show the survival time of the rats as in d. f Western blotting analysis to show protein expression and phosphorylation of intestinal epithelium of rats with sham operation that were administered with vehicle (n = 15) and TCI rats that were administered with vehicle (n = 12), Asn plus IPA (n = 15) or Asn plus IPA and MK-2206 (n = 13). g–m The ELISA to detect indicator concentrations of rats as in f. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001, ns non-significant. TCI traumatic colon injury, Asn asparagine, IPA 3-indolepropionic acid, IL-6 interleukin-6, TNF-α tumor necrosis factor-α, DAO diamine oxidase, mTOR mammalian target of rapamycin, CREB cAMP-response element binding protein, ELISA enzyme-linked immunosorbent assay, POD post-operative day