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Fig. 4 | Military Medical Research

Fig. 4

From: Intracellular accumulation of tau inhibits autophagosome formation by activating TIA1-amino acid-mTORC1 signaling

Fig. 4

Inhibiting the amino acid pathway by ConA attenuates tau-induced mTORC1 upregulation. a Reduction in phosphorylated 4EBP1, p70S6K1 and ULK1 was detected by Western blotting in HEK293T cells, which were treated with different concentrations of concanamycin A (ConA, n = 6). b Application of ConA in HEK293T cells attenuated tau-induced mTORC1 upregulation, as evidenced by the decreased colocalization of LAMP1 (Red) and mTOR (Green), scale bar = 20 μm. The arrows point to colocalization of LAMP1 and mTOR. c Application of ConA in HEK293T cells attenuated tau-induced mTORC1 upregulation, as evidenced by the attenuated levels of p-4EBP1 and p-p70S6K1 (n = 6). d Application of ConA attenuated tau-induced impairment of autophagosomes formation in primary cultured hippocampal neurons, as imaged by transmission electron microscopy, scale bar = 1 μm. The yellow arrows point to the autophagosomes. 4EBP1 eukaryotic translation initiation factor 4E-binding protein 1, GFP green fluorescent protein, LAMP1 lysosome-associated membrane glycoprotein 1, mTOR the mammalian target of rapamycin kinase, ns non-significant, p70S6K1 70 kDa ribosomal protein S6 kinase 1, ULK1 unc-51-like autophagy-activating kinase 1, Vec vector. All data are expressed as the mean ± SEM. **P < 0.01, ***P < 0.001

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