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Fig. 6 | Military Medical Research

Fig. 6

From: Identification of antimalarial targets of chloroquine by a combined deconvolution strategy of ABPP and MS-CETSA

Fig. 6

Functional verification of the CQ target proteins. CQ inhibits the enzymatic activities of the recombinant PfLDH (a), PfOAT (b), PfPyrK (c), PfPGK (d) and PfTPI (e) proteins in vitro in a dose-dependent manner. f Immunofluorescence staining experiment of CQP (2 µmol/L) as well as the target proteins (by respective antibody) and the mitochondria (by MitoTracker, 300 nmol/L). g Co-localization analysis with Pearson's coefficient and Overlap coefficient. LDH L-lactate dehydrogenase, OAT ornithine aminotransferase, PyrK pyruvate kinase, PGK phosphoglycerate kinase, TPI triosephosphate isomerase, DAPI 4',6-diamidino-2-phenylindole, CQP chloroquine analog probe, TAMRA carboxytetramethylrhodamine, Mito mitochondria, Conc. concentration, a.u. absorbance unit

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Military Medical Research

ISSN: 2054-9369