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Fig. 5 | Military Medical Research

Fig. 5

From: Identification of antimalarial targets of chloroquine by a combined deconvolution strategy of ABPP and MS-CETSA

Fig. 5

Binding verification of the CQ target proteins. a CQP specifically binds to recombinant PfLDH, PfOAT, PfPyrK, PfPGK, and PfTPI proteins in a dose-dependent manner. b CQP labels recombinant parasite proteins only under UV irradiation. c Pre-treatment of excess CQ (50×) can compete with the CQP binding to the target proteins. d Validation of the specific targeting of CQP to the 5 target proteins in live parasites by western blot. e-i The determination of binding affinity of CQ with the 5 target proteins through SPR assay. CQ chloroquine, CQP chloroquine analog probe, Flu fluorescence, Coo coomassie, Pf Plasmodium falciparum, LDH L-lactate dehydrogenase, OAT ornithine aminotransferase, PyrK pyruvate kinase, PGK phosphoglycerate kinase, TPI triosephosphate isomerase

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Military Medical Research

ISSN: 2054-9369