Fig. 4
From: Down-regulation of Risa improves podocyte injury by enhancing autophagy in diabetic nephropathy
Risa suppresses autophagy and aggravats podocyte injury via Sirt1-autophagy axis. The HG-induced MPCs were cotransfected with Risa-LV and EX-527. The levels of phosphorylated Sirt1 and GSK3β were examined by Western blotting (a) and immunofluorescence staining (b). c Expression of NPHS2 and Desmin was assessed by Western blotting. d Expression of WT-1 and Nephrin was observed by immunofluorescence staining. e Levels of Beclin-1 and p62 were examined by Western blotting. f Levels of LC3B and p62 were assessed by immunofluorescence staining. MPCs mouse podocyte cells, Risa lncRNA AK044604, GAPDH glyceraldehyde-3-phosphate dehydrogenase, GSK3β glycogen synthase kinase 3β, HG high glucose, HM high mannitol, NG normal glucose, LV lentivirus, Sirt1 sirtuin 1