Fig. 2

Effects of Drp1 intervention on p62-mediated autophagosome formation after CIRI and ODG/R. a Immunofluorescence images showing co-location of p62 and mitochondria (COX IV) in the cerebral cortex after CIRI and Mdivi-1 treatment (bar = 20 μm; n = 5/group). b LC3 II/I and the protein expression of p62 in total, cytoplasmic, and mitochondrial fractions after CIRI and Mdivi-1 treatment. β-actin, COX IV, and tubulin were used as internal references for total, mitochondrial, and cytoplasmic fractions, respectively (n = 8/group). c Confocal images showing co-location of LC3, p62, and mitochondria in OGD/R-treated SH-SY5Y cells after Drp1 shRNA (bar = 25 μm; n = 5/group). d LC3 II/I and protein expression of p62 in total, cytoplasmic, and mitochondrial fractions in OGD/R-treated SH-SY5Y cells after Drp1 shRNA. β-actin, COX IV, and tubulin were used as internal references for total, mitochondrial, and cytoplasmic fractions, respectively (n = 8/group). e Confocal images showing SH-SY5Y cells labeled with Drp1-GFP, p62-RFP, and MitoTracker after Drp1 shRNA (bar = 25 μm; n = 5/group). ^*P < 0.05, compared with normal group; ^#P < 0.05, compared with CIRI or OGD/R group. CIRI cerebral ischemia–reperfusion injury, OGD/R oxygen–glucose deprivation/reoxygenation treatment, Scr. scramble-shRNA