Fig. 3
From: Celastrol mitigates inflammation in sepsis by inhibiting the PKM2-dependent Warburg effect
Identification of potential target proteins of celastrol (Cel) through ABPP combined with LC–MS/MS. a Chemical structure of Cel and celastrol-probe (Cel-P). b Dose-dependent inhibition of RAW264.7 cell proliferation by Cel and Cel-P. c Dose-dependent labeling of proteins by Cel-P in LPS-activated macrophages. d Competition between Cel and Cel-P for protein binding in situ (red star: 58 kD, green star: 43 kD, blue star: 37 kD, yellow star: 25 kD). e Imaging of intracellular Cel-P. Scale bar = 100 μm. ABPP activity-based protein profiling, LC–MS/MS liquid chromatography – tandem mass spectrometry, Fluo fluorescence, CBB coomassie brilliant blue