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Fig. 3 | Military Medical Research

Fig. 3

From: N6-methyladenosine modification of CENPK mRNA by ZC3H13 promotes cervical cancer stemness and chemoresistance

Fig. 3

CENPK promotes cervical cancer stemness, chemoresistance, metastasis, and proliferation. Tumorsphere formation (a) and immunofluorescence assays (b) were adopted to measure stemness of CENPK-depleted HeLa and SiHa cells and the control cells. c Clonogenic assays were adopted to estimate chemoresistance of CENPK-depleted HeLa and SiHa cells and the control cells treated with cisplatin and carboplatin. d Transwell assays were performed to elucidate the migration and invasion of CENPK-silenced HeLa and SiHa cells and the control cells. MTT assays (e), and colony-formation assays (f) were applied to evaluate the proliferation of CENPK-silenced HeLa and SiHa cells and the control cells. g Immunofluorescence was adopted for detecting the expression of γ-H2AX (Ser139) in CENPK-silenced HeLa treated with cisplatin (10 μmol/L for 24 h), CENPK-silenced SiHa cells treated with carboplatin (100 μmol/L for 24 h), and control cells. h EdU incorporation assays were used for elucidating DNA replication of CENPK-depleted HeLa and SiHa cells and the control cells. i Western blotting analysis of the expression of proteins associated with stemness (c-Myc), DNA damage repair (p53), epithelial-mesenchymal transition (Vimentin), and DNA replication (p21, CCND1 and c-Jun) in CENPK-depleted HeLa and SiHa cells and control cells. Data are represented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001

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