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Fig. 1 | Military Medical Research

Fig. 1

From: N6-methyladenosine modification of CENPK mRNA by ZC3H13 promotes cervical cancer stemness and chemoresistance

Fig. 1

ZC3H13 participates in regulating m6A modification of CENPK mRNA. a Correlogram displaying the link between CENPK and 21 m6A regulators expression as per the TCGA CESC dataset. The red frame emphasizes the correlation between CENPK and 21 m6A regulators expression. b Relationship between CENPK and ZC3H13 expression as per the TCGA CESC dataset. c qPCR and Western blotting determining the effect of ZC3H13 knockdown on CENPK expression. d MeRIP assays were adopted to measure the m6A modification of CENPK mRNA. The enriched RNAs were subjected to reverse transcription and further analyzed by qPCR and PCR. e Bioinformatics analysis predicting m6A sequences within the CENPK 3′-UTR. f Luciferase reporter assays were carried out for validating the effect of ZC3H13 knockdown on the post-transcriptional repression of CENPK in HeLa and SiHa cells. Data are represented as the mean ± SD. **P < 0.01; ***P < 0.001; ns non-significant

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